Molecular Characterization Of Phosphate Solubilizing Bacteria (PSB) And Plant Growth Promoting Rhizobacteria (PGPR) From Pristine Soils

Abstract

Plant growth-promoting bacteria (PGPB) are associated with many plant species and are commonly present in many environments. The most widely studied group of PGPB are plant growth-promoting rhizobacteria (PGPR) colonizing the root surfaces and the closely adhering soil interface, the rhizosphere. Free-living PGPR have shown promise as biofertilizers. In addition, phosphorous solubilizing bacteria (PSB) are important for crop plants as they increase phosphorous uptake and play a crucial role as PGPR in the Crop growth enhancement. A total of 53 isolates were isolated from forest soils of Siruvani Hills. The plant growth promoting and phosphate solubilizing efficacy of these isolates was determined under in-vitro conditions. Ribotyping of the 16S rDNA of the promising PSB isolates was performed and the genetic variability within the strains was analyzed by comparing the sequence through BLAST homology and CLUSTAL W2 analysis.